m4018 strain  (Pfizer Inc)

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Circos plots of Icarus contig alignments of S. rimosus M4018 ( A ) and R6-500 ( B ) assemblies against S. rimosus ATCC 10970 reference and comparison of the location of BGCs on the chromosome of S. rimosus ATCC 10970 ( C ), M4018 ( D ), and R6-500 ( E ) strains. Chromosome and plasmid (black arrows) assemblies were evaluated with ATCC 10970 set as the reference sequence. Outer circle—ATCC 10970 chromosome and plasmid, inner circle—sequence coverage of R6-500 ( A ) and M4018 ( B ). Terminal regions of M4018 that we suspect to be duplicated due to double read-depth of PacBio sequences are displayed (blue arrows), while the terminal inverted repeat of R6-500 is also indicated (yellow arrow); this is present at both ends of our R6-500 assembly. The location of the OTC BGC (No. 9) is marked in red. The duplicated region at the chromosome arms of strain R6-500 ( E ) and translocated region in M4018 ( D ) are marked in yellow.

Article Snippet: As both M4018 and R6-500 strains had significantly greater oxytetracycline titers than the ancestral strain ATCC 10970, we first sought to determine the genomic changes that had occurred during the strain improvement programs carried out by Pfizer and PLIVA, respectively.

Techniques: Comparison, Plasmid Preparation, Sequencing

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Schematic presentation of the end of the chromosome containing OTC BGC in S. rimosus ATCC 10970. ( A ) Chromosome of ATCC 10970 with marked BGCs (Table S1). ( B ) Right end of the chromosome, containing OTC BGC. The extent of the deletion present in M4018 is shown (yellow) ( C ), and the locations of the deletions introduced in engineered strains of ATCC Δ145kb and ATCC Δ240kb are in blue ( D1 and D2 ).

Article Snippet: As both M4018 and R6-500 strains had significantly greater oxytetracycline titers than the ancestral strain ATCC 10970, we first sought to determine the genomic changes that had occurred during the strain improvement programs carried out by Pfizer and PLIVA, respectively.

Techniques:

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Production of OTC by industrial strains M4018 and R6-500 and engineered strains with 145 and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.

Article Snippet: As both M4018 and R6-500 strains had significantly greater oxytetracycline titers than the ancestral strain ATCC 10970, we first sought to determine the genomic changes that had occurred during the strain improvement programs carried out by Pfizer and PLIVA, respectively.

Techniques:

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Circos plots of Icarus contig alignments of S. rimosus M4018 ( A ) and R6-500 ( B ) assemblies against S. rimosus ATCC 10970 reference and comparison of the location of BGCs on the chromosome of S. rimosus ATCC 10970 ( C ), M4018 ( D ), and R6-500 ( E ) strains. Chromosome and plasmid (black arrows) assemblies were evaluated with ATCC 10970 set as the reference sequence. Outer circle—ATCC 10970 chromosome and plasmid, inner circle—sequence coverage of R6-500 ( A ) and M4018 ( B ). Terminal regions of M4018 that we suspect to be duplicated due to double read-depth of PacBio sequences are displayed (blue arrows), while the terminal inverted repeat of R6-500 is also indicated (yellow arrow); this is present at both ends of our R6-500 assembly. The location of the OTC BGC (No. 9) is marked in red. The duplicated region at the chromosome arms of strain R6-500 ( E ) and translocated region in M4018 ( D ) are marked in yellow.

Article Snippet: These were strains M4018 (Pfizer) ( ) and R6 (PLIVA) ( , ), both derived independently from S. rimosus ATCC 10970.

Techniques: Comparison, Plasmid Preparation, Sequencing

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Schematic presentation of the end of the chromosome containing OTC BGC in S. rimosus ATCC 10970. ( A ) Chromosome of ATCC 10970 with marked BGCs (Table S1). ( B ) Right end of the chromosome, containing OTC BGC. The extent of the deletion present in M4018 is shown (yellow) ( C ), and the locations of the deletions introduced in engineered strains of ATCC Δ145kb and ATCC Δ240kb are in blue ( D1 and D2 ).

Article Snippet: These were strains M4018 (Pfizer) ( ) and R6 (PLIVA) ( , ), both derived independently from S. rimosus ATCC 10970.

Techniques:

Journal: mSystems

Article Title: Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus

doi: 10.1128/msystems.00250-24

Figure Lengend Snippet: Production of OTC by industrial strains M4018 and R6-500 and engineered strains with 145 and 240 kb deletion compared to the parent strain S. rimosus ATCC 10970. Mean with error bars showing s.d. ( n = 9, three independent fermentations from three biological replicates). Significance is tested with Dunnett’s T3-test, **** P < 0.0001.

Article Snippet: These were strains M4018 (Pfizer) ( ) and R6 (PLIVA) ( , ), both derived independently from S. rimosus ATCC 10970.

Techniques: